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Fluorescence and phosphorescence

Fluorescence and Phosphorescence - Chemistry LibreText

Fluorescence and phosphorescence are types of molecular luminescence methods. A molecule of analyte absorbs a photon and excites a species. The emission spectrum can provide qualitative and quantitative analysis Both fluorescence and phosphorescence are forms of photoluminescence. In a sense, both phenomena cause things to glow in the dark. In both cases, electrons absorb energy and release light when they return to a more stable state. Fluorescence occurs much more quickly than phosphorescence Both fluorescence and phosphorescence are spontaneous emissions of electromagnetic radiation. The difference is that the glow of fluorescence stops right after the source of excitatory radiation is switched off, whereas for phosphorescence, an afterglow with durations of fractions of a second up to hours can occur [6,7] Fluorescence and phosphorescence Possible de-excitation pathways of excited molecules. Various parameters influence the emission of fluorescence. Fluorescence: a process in which a part of energy (UV, Visible) absorbed by a substance is released in the form of light as long as the stimulating radiation is continued

Fluorescence is short-lived with luminescence ending almost immediately. Phosphorescence involves change in electron spin and may endure for several seconds. In most cases, photoluminescent radiation tends to be at longer wavelengths than excitation radiation. Chemiluminescence is based on an excited species formed by a chemical reaction The key difference between fluorescence and phosphorescence is that Fluorescence stops as soon as we take away the light source whereas phosphorescence tends to stay little longer even after the irradiating light source is removed. When a molecule or atom absorbs energy, it can undergo various changes

Fluorescence Versus Phosphorescence - ThoughtC

  1. Phosphorescence Phosphorescence is related to fluorescence in terms of its general mechanism but involves a slower decay
  2. Fluorescence et Phosphorescence. Par Prof. Maurice Curie. (Actualités scientifiques et industrielles, 1010.) Pp. 212. (Paris: Hermann et Cie., 1946.) 350 francs
  3. escence) the photon has effectively no influence on the spin state. Singlet transitions are spin allowed, but can be symmetry forbidden
  4. ated by a UV lamp, sometimes called a black light. Some rocks also glow in black light, the particular colors being a function of the rock's

What are Fluorescence and Phosphorescence? :: Education

Both fluorescence and phosphorescence are type of photoluminescence which involves absorption of energy and excitation of atom to higher energy level followed by emission of electromagnetic radiation (or return to low energy state) In fluorescence, the emission of light stops once the source of the higher energy electromagnetic waves ceases. It means when the source is cut down, fluorescence stops immediately whereas phosphorescence continues. The lifespan of the excited atoms in fluorescence has a very short lifespan. They go back to their normal state very fast Fluorescence and phosphorescence are two forms of photoluminescence. In photoluminescence, a substance's glow is triggered by light, in contrast to chemiluminescence, where the glow is caused by a chemical reaction There are various definitions of fluorescence and phosphorescence with the simplest being that fluorescence is prompt photoluminescence that occurs very shortly after photoexcitation of a substance, while phosphorescence is long-lived photoluminescence that continues long after the photoexcitation has ceased 3.2 Fluorescence Once a molecule arrives at the lowest vibrational level of an exited singlet state, it can do a number of things, one of which is to return to the ground state by photon emission. This process is called uorescence . The lifetime of an excited singlet state is approximately 10 9 to 10 7 sec and therefore the decay time of.

  1. La fluorescence est l'émission de lumière par une substance ayant absorbé de la lumière ou un autre rayonnement électromagnétique, tandis que la phosphorescence désigne la lumière émise par une substance sans combustion ni chaleur perceptible
  2. So phosphorescence and fluorescence are closely related. They both absorb light at certain frequencies, and then radiate it back out at other frequencies. The difference is that phosphorescent materials take their time about it and release their light slowly, while fluorescent materials send their light back out right away
  3. Fluorescence and Phosphorescence 3.4 Phosphorescence and Intersystem Crossing Although population of triplet states by direct absorption from the ground state is insigniflcant, a more e-cient process exists for population of triplet states from the lowest excited singlet state in many molecules. This proces
  4. escence 190 Near-Infrared Fluorescence 192 Fluorescent Nanoparticles 193 Fluorescence-Based Plasmonics 195 Author Information 195 Corresponding Author 195 Notes 195 Biographies 195 Acknowledgments 197 References 197 F luorescence, Phosphorescence, and.
  5. Time-resolved fluorescence anisotropy (TFA) is sensitive to rotational correlation times in the nanosecond to microsecond time range (as is conventional EPR), while time-resolved phosphorescence anisotropy (TPA) is sensitive to microsecond-to-millisecond correlation times (as is saturation transfer EPR)
  6. g, in Encyclopedia of Spectroscopy and Spectrometry (Third Edition), 2017 Phosphorescence Phosphorescence is emission of light from triplet-excited states, in which the electron in the excited orbital has the same spin orientation as the ground-state electron
  7. ate (right), in visible light, in darkness, and after 4

Main Difference The main difference between the fluorescence and the phosphorescence is that fluorescence occurs and cease quickly whereas phosphorescence takes longer time to glow and cease slowly after the removal of the source. Fluorescence Vs. Phosphorescence Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter wavelength, a phosphorescent substance will glow, absorbing the light and reemitting it at a longer wavelength. Unlike fluorescence, a phosphorescent material does not immediately reemit the radiation it absorbs Spectrally Tunable Solid State Fluorescence and Room-Temperature Phosphorescence of Carbon Dots Synthesized via Seeded Growth Method. Jinyang Zhu, Jinyang Zhu. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012 China.

Difference Between Fluorescence and Phosphorescence

The spike at 0 ms is the fluorescence decay which occurs almost instantaneously on this timescale and the longer component is the phosphorescence. The ratio between the fluorescence and phosphorescence at 482 nm can be calculated by integrating the area of the fluorescence (green) and phosphorescence (pink), and therefore, Phosph/Fluor=0.241 Molecular Fluorescence and Phosphorescence Spectra. To appreciate the origin of molecular fluorescence and phosphorescence, we must consider what happens to a molecule following the absorption of a photon. Let's as- sume that the molecule initially occupies the lowest vibrational energy level of its electronic ground state When we talk about luminous watches and paint, what we really mean is phosphorescence, which is very similar to fluorescence: the process by which energy-saving lamps make light. Photo: An energy-saving compact fluorescent lamp (CFL). The fluorescent chemical is a kind of chalky white coating on the inside of the thin glass tubes Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects. Fluorescence and phosphorescence analysis: principles and applications by David M. Hercules, 1966, Interscience Publishers edition, in Englis

spectroscopy - Fluorescence and phosphorescence Britannic

Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors (fluorescence spectroscopy), fluorescent labeling, dyes, biological detectors, cosmic-ray detection, and fluorescent lamps. Phosphorescence. Phosphorescence is a type of photoluminescence related to fluorescence Both fluorescence and phosphorescence lifetimes are therefore more related to Z than to density (Fig. 3). Finally, our synthetic strategy provides a unique opportunity to create materials with. Answer (1 of 8): Both of these processes involve similar interactions with light or other types of electromagnetic radiation. They both require an energy level that a species can 'jump' up to when absorbing radiation, and a ground energy level for the species to relax back down. The ground ener.. 1.3.5 Delayed Fluorescence Delayed fluorescence is a non-collision energy transfer process. It has the fluorescence characteristic emis-sion spectrum but a lifetime shorter than phosphorescence. The intensity of delayed fluorescence is pro-portional to the square of the intensity of the exciting radiation and the square of the phosphorescence

Fluorescence and Phosphorescence Natur

terminology - Fluorescence and phosphorescence - Physics

Fluorescence and Phosphorescence. A uranium glass marble and a rhomb of calcite respectively fluoresce and phosphoresce on a small shortwave (UVC) ultraviolet light. The uranium glass marble exhibits a yellow-green fluorescence. This type of calcite exhibits blue phosphorescence when stimulated with shortwave UV light: it will continue to glow for a few seconds after the UV source is removed Both fluorescence and phosphorescence occur when molecule-bound electrons absorb photons which causes them to excite and enter a higher molecular energy state. Following excitation, the electrons. Fluorescence Microscopy Basic Concepts in Fluorescence. Fluorescence is a member of the ubiquitous luminescence family of processes in which susceptible molecules emit light from electronically excited states created by either a physical (for example, absorption of light), mechanical (friction), or chemical mechanism. Generation of luminescence through excitation of a molecule by ultraviolet. example, S1, to the ground state with the emission of fluorescence can take place easily and within 10-9 - 10-6 seconds, the transition from an excited triplet state to the ground state with the emission of phosphorescence requires at least 10-4 the ground state It is shorter for Fluorescence 10 to 10 -7 s and much longer for Phosphorescence up to a few hours and even days. The effect is widely used in such everyday practical applications as industrial and residential lightning neon and fluorescent lamps as an analytical technique in science and as a quality and process control method in industry

The Pd complex PdN3N exhibits an unusual dual emission of room-temperature phosphorescence (RTP) and thermally activated delayed fluorescence (TADF), but the mechanism is elusive. Herein, we employed both density functional theory (DFT) and time-dependent DFT (TD-DFT) methods to explore excited-stat Fluorescence occurs for longer times than phosphorescence. The feature that distinguishes fluorescence from phosphorescence is the index of refraction n of a material. The feature that distinguishes fluorescence from phosphorescence is the amount of the absorbed energy

Journal of Fluorescence. Journal of Fluorescence is an international forum for peer-reviewed original articles that advance the practice of this established spectroscopic technique. Coverage includes advances in theory and data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, advances in. PHOTOLUMINESCENCE: FLUORESCENCE & PHOSPHORESCENCE Fluorescence and phosphorescence are two forms of photoluminescence. Photoluminescence is the emission of light arising from excited electronic states following absorption of light Many important applications based on photoluminescence have been developed 7

Difference Between Fluorescence and Luminescence Mechanism. Luminescence refers to any mechanism where photons are generated, without an input of heat.. Fluorescence refers to a specific type of luminescence where the energy to produce the photon comes from the absorption of a photon with higher energy. An excited singlet state is produced in the intermediate stages Fluorescence is a type of luminescence too. Electrons in an atom or a molecule can absorb the energy in the electromagnetic radiation and thereby excite to an upper energy state. This upper energy state is unstable; therefore, electron likes to come back to the ground state Afterglow including thermally activated delayed fluorescence (TADF) and room-temperature phosphorescence (RTP) has stimulated considerable attention owing to bright potential applications in optoelectronic devices, sensing, and security systems. However, previously reported afterglow materials are mostly si Accordingly, they exhibit bright blue-green phosphorescence at low temperatures (<100 K), which turns into delayed fluorescence by increasing the temperature. However, unusually, the fluorescence is practically absent in two copper complexes which otherwise still conform to the TADF scheme The terms 'fluorescence' and 'phosphorescence' are often used rather loosely by non-specialists. 'Phosphorescence' tends to be used to describe any long-lived emission, for example the glow emitted by phosphorus as it oxidises in air (this is actually chemiluminescence). Some long-lived emission is actually fluorescence that is emitted as a.

Video: Fluorescence and Phosphorescence Atomic Physic

98% (55 ratings) Transcribed image text: For a given fluorophore, choose the answer from the following options that correctly lists the processes of fluorescence, absorption, and phosphorescence in order from shortest to longest wavelength. O absorption, fluorescence, phosphorescence O fluorescence, phosphorescence, absorption O phosphorescence. A fluorescence microscope is used to study organic and inorganic samples. Fluorescence microscopy uses fluorescence and phosphorescence to examine the structural organization, spatial distribution of samples. It is particularly used to study samples that are complex and cannot be examined under conventional transmitted-light microscope

Phosphorescence is a physical phenomenon closely related to fluorescence. The main criterion that is used to distinguish between them is the length of emission radiation. While during fluorescence excited molecules emit light instantly (<=10 -8 s), during phosphorescence, excited molecules undergo a transition to a lower lying metastable state. 1. the visible or invisible radiation emitted by certain substances as a result of incident radiation of a shorter wavelength such as X-rays or ultraviolet light. the property of absorbing light of short wavelength and emitting light of longer wavelength 4.1 Fluorescence Spectroscopy for concentration determination. Figure 8: Calibration curve from spectra. does not work well with high concentrations. inner filter effect (two ways): surface facing molecules absorb all of the light. if overlap between excitation and emission, emission can be re-absorbed by analyte 1.1 After the Excited State, Fluorescence Appears As Light Energy. 1.2 Fluorescence Spectra Different from the Excitation Light Are Measured at Long Wavelengths. 1.3 The Measurement Method Is a Two-Peak Observation Including Excitation Spectra. 2 Phosphorescence Is a Long-Lasting, Weakly Fluorescent Phenomenon Myth: Bioluminescence is the same as fluorescence (or phosphorescence, or chemiluminescence). FACT: All of the terms apply to the production of light from chemicals, but bioluminescence is only similar to chemiluminescence. In fluorescence, the energy from an external source of light is absorbed and almost immediately reëmitted

• Unlike fluorescence, a phosphorescent material does not immediately re-emit the radiation it absorbs • Phosphorescence is a process in which energy absorbed by a substance is released relatively slowly in the form of light. • This is in some cases the mechanism used for glow-in-the-dark materials which are charged by exposure to light whether fluorescence determinations were affected by the presence of the phosphores-cence probe and whether phosphorescence evaluations were affected by the presence of FITC. Figure 1 schematically illustrates the sys-tem used to measure fluorescence and phos-phorescence in small regions. All studies were performed using a Leitz Orthoplan mi Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence. Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter wavelength, a phosphorescent substance will glow, absorbing the light and reemitting it at a longer wavelength

7 Differences between Fluorescence and Phosphorescenc

Fluorescence occurs much more quickly than phosphorescence. What is the difference between phosphorescence and fluorescence? Biofluorescence is the absorption of wavelengths from the visible light spectrum by fluorescent proteins in a living organism and the emission of light at a lower energy level (a different color light) Fluorescence due to the following reasons: (1) Phosphorescence is extensively quenched by oxygen under normal experimental conditions (i.e., oxygenated samples at room temperature) (2.

Fluorescence - Explanation, Examples and FAQ

  1. eral substances being investigated by George G. Stokes in 1852, hence the name fluorescence given at the phenomenon. Fluorite is a reservoir for many of the rare earth elements
  2. eralogy, gemology, chemical sensors (Fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, and, most commonly, fluorescent lamps. source Phosporescence Phosphorescence is a specific type of photolu
  3. These visual phenomena bring together science and nature and go by the names of fluorescence and phosphorescence. FLUORESCENCE. Fluorescence produces vivid colors when an ultraviolet light source is directed at a gemstone. Each gem behaves differently when exposed to ultraviolet light - those that are affected by the light in return fluoresce.

Phosphorescence. Phosphorescence is the emission of light in a similar manner to fluorescence but on a longer timescale, so that emission continues after excitation ceases. The cover photo shown below is an example of bioluminescence (from a Dino-Tile) emanating from the lower right corner of the Dino-Tile Fluorescence: Gems and Minerals Under Ultraviolet Light, Manuel Robbins, 1994, Geoscience Press, ISBN -945005-13-X , The World of Fluorescent Minerals, Stuart Schneider, Schiffer Publishing, 2006, ISBN -7643-2544-2 , Handbook of Fluorescent Gems and Minerals, a practical guide for the gem and mineral collector, Jack de Ment, 1949 Fluorescence, Phosphorescence, Description: Excitation of e- by absorbance of hn. - Re-emission of hn as e- goes oxidize compound ' paramagnetic property increase intersystem crossing (spin flipping) - PowerPoint PPT presentation. Number of Views: 326. Avg rating:3.0/5.0 phosphorescence emission and short-lived fluorescence emission, both of which would originate from the same sample. • Both mechanical and electronic devices are used, and many commercial fluorescence instruments have accessories for phosphorescence measurements. Many of the current instruments use a gated scheme for the delay Fluorescence is the property of some atoms and molecules to absorb light at a particular wavelength and to subsequently emit light of longer wavelength after a brief interval, termed the fluorescence lifetime. The process of phosphorescence occurs in a manner similar to fluorescence, but with a much longer excited state lifetime

Fluorescence and Phosphorescence from Individual C 60 Molecules Excited by Local Electron Tunneling Elizabeta Ćavar, Marie-Christine Blüm, Marina Pivetta, François Patthey, Majed Chergui, and Wolf-Dieter Schneider Phys. Rev. Lett. 95, 196102 - Published 3 November 200 WHAT ARE FLUORESCENCE AND PHOSPHORESCENCE? HOW DO THEY WORK? This activity was presented as part of the Optics Magic @Home series of webinars, hosted by OSA, the Optical Society. Spanish translations are not available for this lesson. MATERIALS Activity 1 - Fluorescence An ultraviolet light. This can be a keychain UV-LED, or a UV-LED flashlight [ Phosphorescence: Fluorescence: Fig. 3: Simultaneous fluorescence and phosphorescence lifetime imaging The procedure can be further refined by using the laser on/off information as a routing signal to better separate the fluorescence in laser-on phases from the phosphorescence in the laser-off phases, please see [6, 7, 12] Both fluorescence and phosphorescence are based on the ability of a substance to absorb light and emit light of a longer wavelength and therefore lower energy. The main difference is the time in which it takes to do so.. Evaluate your knowledge of fluorescence and phosphorescence with this multiple-choice quiz and worksheet. The five questions in this quiz require you to know how to determine the concentration of.

What is the difference between fluorescence

Lecture Demonstrations of Fluorescence and Phosphorescence. David P. Richardson* and Raymond Chang. Department of Chemistry, Williams College, Will iamstown, MA 01267, David.P.Richardson@williams. Answer: Incandescence - emission of light (visible electromagnetic radiation ) as result of temperature( kinda thermal radiation ) Fluorescence -emission of light by a substance on absorbing electromagnetic radiation or light.( on electronic bombardment of ,electrons gain energy and excites to h.. phosphorescence, emission of light from a substance exposed to radiation and persisting as an afterglow after the exciting radiation has been removed.Unlike fluorescence, in which the absorbed light is spontaneously emitted about 10-8 second after excitation, phosphorescence requires additional excitation to produce radiation and may last from about 10-3 second to days or years, depending on. We present a lifetime imaging technique that simultaneously records the fluorescence and phosphorescence lifetime images in confocal laser scanning systems. It is based on modulating a high-frequency pulsed laser synchronously with the pixel clock of the scanner, and recording the fluorescence and phosphorescence signals by multidimensional time-correlated single photon counting board

In this Special Issue devoted to fluorescence and phosphorescence in OLED devices, chemists, physicists, material scientists, and electronic and process engineers will find in-depth coverage on organic materials used in OLED technology, from basic concepts to technological and industrial aspects. Mr. Geffroy Bernard Dr. Denis Tondelier Guest Edito complex'-aproductthatdoesnotfluoresce.Fluorescence intensitydecreases withsteadystatequenchingas: 6 36 where K s is the association constant of the quencher and the fluorophore. Fluorescence lifetime is not affected by steady state quenching as the excited states are not involved. Phosphorescence. Fluorescence and Phosphorescence - PHRM 2041. STUDY. Flashcards. Learn. Write. Spell. Test. PLAY. Match. Gravity. Created by. Sl26. Terms in this set (9) What is fluorescence and phosphorescence? It is the release of energy in the form of light. What types of molecules can fluoresce? (3) 1. Rigid molecules 2. Molecules which can absorb light. Delayed fluorescence Decay Curve FL8500. Conclusion Both the FL6500 and FL8500 fluorescence spectrometers are suitable for measuring delayed fluorescence and phosphorescence lifetimes. The pulsed xenon lamp of the FL6500 gives excellent control over the detection parameters and makes it the ideal choice for this application

Fluorescence, Phosphorescence and Photoluminescence

Phosphorescence is a process where the photon is emitted, not from a singlet-excited state, but from a forbidden triplet state. The time scale of emission is generally in the picosecond to nanosecond range, while phosphorescence typically lasts for fluorescence microseconds, milliseconds, or even longerminutes or hours Simultaneous fluorescence and phosphorescence from SPIE. Quenching takes a characteristically elevated temperature because their skin should be studied for a valid file can be drastically by experts to dry ice temperature. Mike West front was used on the PerkinElmer Model 1000 fluorescence spectrometer in 1975 Measurement of Delayed. Fluorescence and phosphorescence are emitted from the sample at all angles, so orientation of the detector at 90 degrees minimizes the source light scattered to the detector. A very useful technique is possible with phosphorescence, since emission can still be detected after the source lamp is turned off Consequently, phosphorescence has a very long excited-state lifetime, reaching hundreds of milliseconds to single-digit seconds. 5, 8 This is between a million and a billion times slower than the spin-allowed fluorescence originating from the singlet state S 1, itself having typical lifetimes in the nanosecond regime

Différence entre la fluorescence et la phosphorescence

fluorescence. In the first case, the laser photon does not have enough energy to excite molecular fluorescence. In the second case, the fluorescence may be excited, but the emission is widely separated in energy from the Raman signal so that the Raman spectrum can be recorded without the fluorescence interference fluorescence and phosphorescence spectroscopy: physicochemical principles and practice. author schulman sg coll. pharm., univ. florida source internation fluorescence nor phosphorescence is seen at neutral or alka- line pH. When the heme is separated, the residual protein exhibits normal emission. In neutral solution the emission is quenched by energy transfer from the excited singlet states of tyrosine and tryptophan to the excited states of the heme..

Fluorescence and Phosphorescence - Andrew Glassne

b) Fluorescence c) Phosphorescence d) Spontaneous emission. Answer: c. Explanation: If the absorption of electromagnetic radiation by matter results in the emission of radiation of same or longer wavelengths for a long time, the phenomenon is termed as phosphorescence. Phosphorescence is a type of luminescence. 4 The division of luminescence into fluorescence and phosphorescence is out of date, since it is an arbitrary distinction based on a qualitative measurement of the duration of luminescence. In terms of the mechanism for converting the excitation energy into visible emission, fluorescence, as a rule, is spontaneous luminescence; hence τ is.

The meaning of PHOSPHORESCENCE is luminescence that is caused by the absorption of radiations (such as light or electrons) and continues for a noticeable time after these radiations have stopped The development of organic luminescent materials with bimodal emissions of both fluorescence and room-temperature phosphorescent (RTP) remains a challenge. The investigation of the relationship between fluorescence and RTP performance is especially rare. In this work, we obtained an organic luminescent molecule, 1,4-phenylenebis((9H-carbazol-9-yl)methanone) (PBCM), which exhibits bimodal. Phosphorescence: If the emission of light continues after the source of exciting radiation is cut off the phenomenon is known as phosphorescence. The period of phosphorescent radiation may be a few seconds or more. Sulphides of alkaline earth metals are good examples of phosphorescent substances. Both phosphorescence and fluorescence are. 1 (888)302-2675 1 (888)814-4206. Unlike other services, these guys do follow paper instructions. It was the first time I didn't have to ask Theory And Interpretation Of Fluorescence And Phosphorescence|Ralph S for a revision. The support and the writer were professional and the paper was delivered 1 day sooner than I expected Phosphorescence Lifetime Imaging (PLIM) is similar to Fluorescence Lifetime Imaging (FLIM), only that it images the phosphorescence from the sample and consequently covers time ranges up to milliseconds. Analogous to FLIM, the contrast in a PLIM image is based on the lifetime of individual fluorophores rather than their emission spectra

Inside the Shadow Box, visitors can create unique shadow art through the process of phosphorescence. What's happening is a bright light is flashing on this wall and the wall starts to glow.

Fluorescent/Phosphorescent DopantsWhat&#39;s Luminescence (animated)Time-Resolved Fluorescence | Fluorescence LifetimePhosphorescence - WikipediaPhosphorescenceFluorimétrie &quot;émission de fluorescence&quot; — Chimie Analytique